Molecular Staging Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
  • Technology

    We plan to develop and commercialize gene-based tests and immunoassays based on our core technology, Rolling Circle Amplification Technology (RCAT™), that can significantly improve the diagnosis of cancer, infectious diseases, neuropsychiatric disorders, auto-immune disorders, and other major disease states.

    How It Works:
    In order to diagnose a disease, physicians often need to look for things that are small in size such as cancer cells, virus particles, bacteria, mutated genes, etc. Physicians will use tests to find “target molecules” associated with these diseased cells or pathogens that indicate their presence. These target molecules might be the DNA of a bacteria or virus, a protein produced by cancerous cells, antibodies to a particular infection, or a particular sequence of a gene mutation. These target molecules are often present at very low levels, particularly in early stages of the disease when treatment might be the most beneficial. Physicians need a test that can easily locate the molecule of interest, and then “amplify” a signal from that molecule to indicate its detection.

    RCAT is a highly sensitive and efficient amplification method that allows the user to detect the presence of target molecules in a wide array of testing formats. It is the only practical amplification method that allows recognition, amplification and detection of targets directly on a solid surface, such as within a cell (in situ analysis) or on a microarray/biochip. It solves several problems inherent in the Polymerase Chain Reaction (PCR), the technology most commonly used to detect genes and their mutations.

    Through access to RCAT™ and related innovations, MSI can:

    • Detect single target molecules or “analytes”
    • Amplify signals from proteins as well as DNA and RNA
    • Pinpoint the location of molecules that have been amplified on a solid surface (in situ analysis/ biochips) since, unlike PCR, the amplified product remains attached to the target molecule.
    • Measure many different targets simultaneously
    • Improve the ease and accuracy of quantitation
    • Simplify haplotype identification through phasing
    • Increase sensitivity with up to 1012 -fold amplification in 1 hour
    • Amplify DNA templates that vary in length from 1 base-pair to over 100 Kilobases
    • Obviate the need for the time-consuming and expensive steps of thermal cycling currently required by most other amplification methods
    • Analyze targets in solution or solid phase.

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