Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • images59Many studies in the yeast Saccharomyces transcriptomic in analyzing the gene expression program in a variety of conditions. Most if not all the cited studies analyzed only the different levels of mRNA by conventional genomic techniques. Indeed, in many cases implicitly assumes that the levels of each mRNA are a direct consequence of the action of one or several transcriptional regulators. This does not take into account that mRNA levels depend both on its rate of synthesis and the degradation. This study, ‘paper of the week “in the journal J. Chem, is a collaboration between two groups at the universities of Lleida and Valencia, under the responsibility of Henry Smith and Jose Enrique Pérez-Ortín, respectively. Read the rest of this entry »

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  • images54Transcription is a process in eukaryotic cells is coupled to mRNA processing and transport. In recent years, we know that the transcription of at least a number of genes occurs at the nuclear periphery near the nuclear pore. In yeast ASaccharomyces cerevisiae proteins Thp1, Sac3 and Cdc31 form a complex (THSC), associated with nuclear pore affecting the process of transcription and RNA transport. This complex is also present Sus1, a protein identified as a component of the SAGA complex, involved in transcription initiation. Read the rest of this entry »

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  • 197-32Research in the past eight years has driven the classical idea that nuclear processes occurring from transcriptional activation to the mature mRNA out of the nucleus were independent events. The work of many groups has contributed significantly to turning this vision of gene expression, and currently accepted model of coupled processes that ensure the proper output for translation of mRNA in the cytoplasm. Read the rest of this entry »

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  • Initiation Apparatus Transcription Initiation Apparatus

    We discovered that the RNA polymerase II transcription initiation apparatus contains a multiprotein complex, called the Srb/mediator complex, which is involved in mediating the response to transcriptional activators and repressors. We have isolated genes encoding components of the yeast, murine and human Srb/mediator complexes and have used them to study the roles of specific Srb/mediator proteins in gene regulation . Read the rest of this entry »

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  • Several themes are featured. Transcriptional activators bind to specific DNA sequences, and the chromatin context of the DNA biding site can have a positive or negative influence on binding of the activator or the proteins it recruits to the promoter. Activators can bind and recruit chromatin remodeling and modifying complexes that influence local chromatin structure. Read the rest of this entry »

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  • vrk-kinases-a-new-signaling-pathway-in-mammalsThree papers were signed at a joint CSIC and the University of Salamanca describe the identification and characterization of a new family of serine-threonine kinases of human carcinoma, in the course of evolution, separated from the branch that subsequently caused the casein kinases. The catalytic domain of this new family VRK (vaccinia-related kinases) is related to the vaccinia virus B1R protein.  Read the rest of this entry »

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