Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
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The specificity of the DNA segments catalyzed by the enzyme DNA ligase can be exploited in systems designed to identify the presence of a target sequence of nucleic acids in a biological sample.
The amplification methods based on the ligase can increase sensitivity without causing a decrease in specificity. Have described modifications of these techniques that reduce non-specific ligation. The automation of testing in the future allow the routine use of such technology in the clinical laboratory.
The basic principle of any molecular diagnostic technology is the detection of a specific sequence of nucleic acids by hybridization with a complementary sequence, the probe, followed by the detection of the hybrid. However, the sensitivity of tests using nucleic acid probes without resorting to amplification is lower than the classical tests. Its primary application is the identification of microorganisms rather than detection. Read the rest of this entry »
The introduction of rapid methods of sequencing in the late ’70s represented a major shift in studies of two methods of DNA sequencing of nucleic acids. One of them (Maxam and Gilbert) chemical reagents used to cut DNA at specific base. The other is called enzymatic chain termination or dideoxy (Sanger, Nicklen and Coulson). The purpose of both is to get the whole sequence of each of the bases that form a nucleic acid fragment. Read the rest of this entry »