Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • 3A rare disease characterized by premature aging is modeled after a group of Spanish scientists to better understand the relationship between cancer, genes and aging. This is Werner syndrome, an inherited disorder characterized by premature aging of the individual, genomic instability and an increased incidence of tumors. The study was conducted jointly by researchers at the Hospital Clinic of Barcelona, Biomedical Research Center of Navarre, the National Institute on Aging U.S., and the group of Manel Esteller of the CNIO. Read the rest of this entry »

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  • images84The gene hedgehog (hh) was initially identified as a key gene in embryonic development of the fruit fly, Drosophila melanogaster. While Drosophila has only one hedgehog gene, vertebrates have three peers. These proteins are secreted extracellular ligands by a group of cells during organ formation in the embryo. In the adult hedgehog maintains and regulates stem cells in many tissues, being instrumental in regenerative processes. There is also a close relation between HH signaling pathway and the development of tumors, genetic alterations observed that stimulate the growth of tumor cells in the digestive tract, lung and prostate .

    And Molecular Genetic studies of this approach has shown its relationship with cholesterol and lipids. Thus, both Drosophila and vertebrates, the active molecule is modified by HH cholesterol, varying the diffusion of HH activity through epithelial tissue and their signaling. In addition, the operation of the route depends on intracellular sterol content, so the appearance of the same type of malformations during pregnancy, in patients deficient in the signaling pathway of HH and in patients with defects in cholesterol synthesis could be caused by the lack of signs of HH.

    This study demonstrates that in Drosophila, hedgehog is transported through the epithelial cells of a lipoprotein in the same way that lipids and cholesterol are transported through the bloodstream. And as expected, the receiver of the road, patched, acts as a receptor of low density lipoproteins (LDLR) and its ability to modulate lipid homeostasis. With these results we get energy metabolism correlate with embryonic development and carcinogenesis, a potential new therapeutic target for the treatment of various cancers.

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  • images42Alzheimer’s disease (AD) and other neurodegenerative processes are characterized by a deregulation of intracellular levels of neuronal Ca 2 +. This alteration seems to carry an implicit cause-effect relationship to toxicity by the β-amyloid peptide and neuronal death, although the mechanisms involved are discussed. One of the systems with higher affinity for Ca 2 + is formed by the Ca 2 +-ATPase. In a study conducted by Ana M. Mata, Department of Biochemistry and Molecular Biology and Genetics, University of Extremadura, have investigated the functional dependence of Ca 2 + ATPase of plasma membranes and intracellular in human brains affected by AD. Read the rest of this entry »

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  • melissa-pvc-sandalia4The group of Roberto Solano, Department of Plant Molecular Genetics at the National Center for Biotechnology, made the study of signaling pathway of the phytohormone jasmonate (JA) in Arabidopsis thaliana and the molecular mechanisms underlying the activation, plant defenses against pathogens.

    It is known that in the perception of JA requires two proteins, both protein coronatina insensitive 1 (COI1) and the JAZ repressor proteins (jasmonate ZIM domain). In a first step regulator, the precursor of JA enzyme binds to molecules such as L-Ile jasmonoil producing the active ligand-isoleucine(JA-Ile). The formation of JA-Ile complex with these proteins leads to ubiquitination and degradation of JAZ proteins allowing, then, the transcription of genes involved in plant development and defense against pathogens. Read the rest of this entry »

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  • images32The specificity of molecular techniques is dependent on the stringency (“stiffness”) of the hybridization conditions, which is determined by a combination of different factors including temperature and salt concentration. The presence of a target sequence in a clinical specimen is demonstrated by the formation of a hybrid between the target nucleic acid and probe. Read the rest of this entry »

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  • images24The hemidesmosomes are multifunctional multiprotein complex of great importance to the integrity of epithelia. Exercise of mediators of cell adhesion and confer resistance to mechanical stress, linking the extracellular matrix with cytokeratin filaments in the cell. They consist of three components transmembrane: α6β4 integrin, BP180-type collagen XVII and integrin-associated tetraspanina CD151. Integrin α6β4 is a receptor for laminin with high specificity for laminin-5, or basal membrane protein that binds to epidermal cytokeratin through plectina and BP230. Read the rest of this entry »

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  • simultaneous-analysis-of-multiple-analytesMany disease states require panels of different tests to be conducted simultaneously (multiplexing) to achieve a clinically complete picture. To date, efforts to multiplex tests have been constrained by issues of background noise or interference, or the requirement for extremely sensitive methods of analyte detection.

    One of the unique features of RCAT is that during the amplification process the signal remains tethered to the analyte of interest. This effectively enables multiplexing capabilities with unprecedented sensitivity and specificity.

    Current applications of RCAT technology have been developed in a multiplexing format enabling greater than 100 different proteins to be measured simultaneously with significantly greater sensitivity than other current methods. Read the rest of this entry »

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  • arabidopsis-thaliana-convenient-relationsViroids are the bottom rung of the biological scale, being exclusively composed of a small circular RNA of 250-400 nucleotides lacking any coding capacity, a key aspect that differentiates them from the viruses that encode proteins themselves. Virus and viroids also have an independent evolutionary origin, has been suggested that the latter are ancient molecules that come from precelulares early development of life on our planet. Read the rest of this entry »

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  • hammerheads-natural-more-efficient-than-their-artificial-derivatives1The hammerhead Ribosome natural, found in some various (small RNA of 250-400 nucleotides able to replicate and cause disease in certain plants), catalyze cis cutting their own oligomeric intermediates generated during replication by rolling circle mechanism. The hammerhead Ribosome, thanks to its structural simplicity, have been manipulated in this study to act in trans as restraining ribonucleases specific RNA, assuming that the similar catalytic behavior in this context is artificial. Read the rest of this entry »

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