Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • images49During the last two decades has been possible to identify the molecular mechanisms that regulate the cell cycle and hence cell division. Accurate knowledge of how a healthy cell divides is useful to understand properly what has not worked in a tumor cell. In this context, the use of a model organism, such as Schizosaccharomyces pombe, has the advantage of a simple and easy genetic manipulation in the laboratory. Moreover, the underlying mechanisms that control the cell cycle are highly conserved through evolution and functioning of these processes appears to be very similar in all eukaryotic organisms. 1.2 Read the rest of this entry »

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  • images43In the laboratory of Fernando Rodriguez Pascual, Researcher, Center for Biological Research of the CSIC, are very interested in studying mechanisms regulating gene expression vasoconstrictor peptide endothelin-1 (ET-1) at both transcriptional and posttranscriptional . In a previous work, the group had reported that certain elements rich in adenine and uridine (AU-rich elements or ARE) present in the 3′-untranslated region (3′-UTR) of this gene contribute to the destabilization of mRNA.
    In the paper published in Molecular and Cellular Biology are proposed to analyze the factors and signaling pathways involved in this process of destabilization. The results have identified the glycolytic enzyme 3-glyceraldehyde phosphate dehydrogenase (GAPDH) as the major protein able to interact with ARE elements in the 3′-UTR of the gene for ET-1. The binding of GAPDH to these elements promotes the rapid degradation of mRNA. The study shows that GAPDH acts as a key sensor of oxidative stress. After a redox stimulus, during which the ratio reduced glutathione / oxidized glutathione decreases GAPDH key cysteine reacts with glutathione to form a mixed disulfide in a process called S-tiolación. Read the rest of this entry »

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  • images13All the talk will focus on what conditions should apply to a laboratory of Molecular Biology, clinical type. While Molecular Biology covers various fields during the exhibition will emphasize that associated with nucleic acids: DNA and RNA.

    After displaying a slide showing the spatial organization of the Molecular Biology Laboratory Diagnostics Ampligen will expose what were the reasons for such distribution, which are absolutely necessary instruments for each of the areas to function autonomously and independent. Read the rest of this entry »

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  • images12In our department we started using molecular biology techniques from the late 80s. Initially we worked with in situ hybridization with DNA probes biopsies with enzymatic labeling, currently only performed in situ hybridization to detect Epstein-Barr virus by PNA probes. In 1989 we added to our laboratory, using materials purchased with a grant DGCYT, hybridization techniques with radioactive probes with which we began conducting a systematic typing of different human papilloma virus. Read the rest of this entry »

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