Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • images18In liquid phase hybridization, HPV probe and target are in solution. The liquid-phase hybridization was used to estimate the genetic relationship among different HPVs, today is of little use.

    The hybrid capture system also comes amid the specimen is denatured líquido.Primero then HPV DNA is hybridized with the corresponding probe. The hybrids are captured by the receptacle, covered with anti-hybrid antibodies that react with alkaline phosphatase, becoming visible after the application of a chemiluminescent substrate. The uptake of hybrid systems are still in experimental phase, initial tests can involve highly sensitive and easy technique. IN LIQUID PHASE

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  • Hybridization solution: The probe and target nucleic acid are in a liquid. The hybridization conditions must be in order. The rate of formation of the duplex under these conditions is high. The problem with this type of hybridization is the elimination of unreacted probe, using among other methods nuclease S1-precipitation with trichloroacetic or hybridization protection assay. Read the rest of this entry »

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