Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • images74La función conocida hasta el momento de la proteína securina es evitar que las cromátidas hermanas se separen prematuramente antes de estar cada una de ellas anclada correctamente a los microtúbulos del huso mitótico. Esta función la realiza la securina interaccionando con la enzima separasa e inhibiendo su actividad proteolítica. Una vez las cromátidas hermanas están dispuestas y ancladas correctamente en el plano ecuatorial, la securina se ubiquitiniza y se degrada, liberando a la separasa, que entonces digiere la cohesina Scc1. La digestión de la proteína Scc1 tiene como consecuencia la desestabilización del complejo proteico que mantiene unidas las cromátidas y permite la distribución de éstas a cada uno de los polos celulares. La separación prematura de las cromátidas puede originar una distribución anómala y la aparición de aneuploidías. Read the rest of this entry »

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  • melissa-pvc-sandalia6Following his doctoral dissertation at the Universidad Autonoma de Madrid, Raul Mendez spent the next eight years of research activity in American laboratories of Robert E. Rhoads (Louisiana State University Medical Center) and Joel D. Richter (University of Massachusetts Medical Center). This postdoctoral experience endorsed joining the brand new Center for Genomic Regulation (CRG) in Barcelona back in 2001, where he went to lead the group, Translational control of gene expression, in the Gene Regulation Program of the center. Read the rest of this entry »

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  • images49During the last two decades has been possible to identify the molecular mechanisms that regulate the cell cycle and hence cell division. Accurate knowledge of how a healthy cell divides is useful to understand properly what has not worked in a tumor cell. In this context, the use of a model organism, such as Schizosaccharomyces pombe, has the advantage of a simple and easy genetic manipulation in the laboratory. Moreover, the underlying mechanisms that control the cell cycle are highly conserved through evolution and functioning of these processes appears to be very similar in all eukaryotic organisms. 1.2 Read the rest of this entry »

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  • images48The cells of any living being divided by two different mechanisms: through mitosis, which is the way in which cells are involved in normal cell growth in most living organisms, and through meiosis, the process by formed sex cells or germ.The meiotic cell cycle differs from the mitotic to have a phase called premeiotic DNA synthesis. This phase is characterized by high levels of recombination and two successive nuclear divisions. REM1 is a cyclin that is expressed only in meiosis in the fission yeast Schizosaccharomyces pombe. Read the rest of this entry »

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  • images45The control of gene expression depends on the access of chromatin gene regulatory areas to the transcriptional machinery. The covalent modifications of histones as acetulaciones and methylation are key factors to regulate that access. Reading the information contained in these posttranslational modifications of histones depends on the function of effector proteins. Therefore, understanding the mechanisms of recognition of these effectors and how histone modification patterns result in complex patterns of expression is key to understanding the transcriptional process. Read the rest of this entry »

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  • lcr1The specificity of the DNA segments catalyzed by the enzyme DNA ligase can be exploited in systems designed to identify the presence of a target sequence of nucleic acids in a biological sample.

    The amplification methods based on the ligase can increase sensitivity without causing a decrease in specificity. Have described modifications of these techniques that reduce non-specific ligation. The automation of testing in the future allow the routine use of such technology in the clinical laboratory.

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  • images21The enzyme DNA ligase is essential for DNA replication, recombination and for repair of genetic material in vivo. From virus and both prokaryotic and eukaryotic organisms have been isolated and characterized different DNA ligases, most recently, have been cloned and purified DNA ligases from thermophilic microorganisms. The ligases characterized to date consist of a single polypeptide chain and exhibit different activities. The ligation activity includes: 1) the binding of double-stranded nucleic acids with cohesive terminals, 2) the joining of blunt-end complex, and 3) the binding of nucleic acid fragments through gap sealing mechanism (nick -sealing). Read the rest of this entry »

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  • images18In liquid phase hybridization, HPV probe and target are in solution. The liquid-phase hybridization was used to estimate the genetic relationship among different HPVs, today is of little use.

    The hybrid capture system also comes amid the specimen is denatured líquido.Primero then HPV DNA is hybridized with the corresponding probe. The hybrids are captured by the receptacle, covered with anti-hybrid antibodies that react with alkaline phosphatase, becoming visible after the application of a chemiluminescent substrate. The uptake of hybrid systems are still in experimental phase, initial tests can involve highly sensitive and easy technique. IN LIQUID PHASE

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  • images17Filter in situ hybridization (HISF). This method also requires DNA extraction and analysis of samples obtained by scraping or swab or brush. The cells are applied directly to a filter which is treated with alkali to cell lysis and DNA denaturation. HISF The method is very easy to implement, fast and inexpensive, however, little specific and often difficult to separate the positive signals from the bottom (1). Its use is limited to work with large numbers of specimens. Read the rest of this entry »

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  • The Chain Reaction (PCR) is a laboratory technique that permits copying in vitro of specific DNA sequences and has revolutionized the field of molecular biology. Conceptually, the PCR is a simple technique is to heat the separation of the two strands of DNA to be amplified is, and a copy simultaneously from a point determined by an artificial DNA fragment called primer, through the action of an enzyme called DNA polymerase. The result is a doubling of the number of molecules of a particular sequence of DNA. This process is repeated a number of times or cycles, such as 30, and achieved an increase or exponential amplification of the number of copies of template DNA fragment. Read the rest of this entry »

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