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- The Molecular Biology of HIV/AIDS
- The epigenetics of cancer, a recent view
- Rolling Circle Amplification Technology–Technical Details
- Structure of an enzyme and its in hibitor
- Development and morphogenesis: potentialities from common patterns
- Cancer as a Disease of the Cell Cycle
- Employment Opportunities
- HYBRIDIZATION METHODS IN LIQUID PHASE
- Human skin analysis
- PROPERTIES OF DNA
- Employment Opportunities at MSI
- The mitofusin 2 in mitochondrial energization
- Apoptotic mechanisms of granzymes in CTL lysis
- Induction therapy of autophagy and apoptosis in melanoma cells
- Parallel evolution of the venom of snakes and integrin
After four years of postdoctoral fellowship at the Cold Spring Harbor Laboratory under the supervision of Carol Greider, the disciple of Margarita Salas, Maria Blasco returning to Spain in 1997 to join the National Center for Biotechnology, CSIC. There, Blasco intended to continue his research work that had enabled him to clone one of the telomerase genes and generated the first knockout mouse for this gene. Now in our country, it happened several works of broad impact on the development of animal models for studying the involvement of telomerase in cancer development and aging processes. Read the rest of this entry »
Chemotaxis is the movement which carried out certain cells in response to a
chemical stimulus. In multicellular organisms, key cells are capable chemotactic
leukocytes. This ability is essential for immune system function and homeostasis
and its deregulation is associated with chronic inflammatory processes.
The movement of leukocytes is the result of an asymmetric
reorganization of their cytoskeleton: At the anterior end, the leading edge
expands due to polymerization of actin-F, while in the uropod retracts back
because of the contraction of actomyosin filaments. This reorganization is
asymmetric, in turn, result in the polarization of different molecules and the
complex signaling cascades that occur in response to chemical stimulation.
Santos Manes’s team at the National Center of Biotechnology, CSIC has conducted
various studies on the molecular mechanisms that cause this cell polarity. On
this occasion, have investigated the role of the enzyme phosphatidylinositol
4-phosphate 5-kinase type I isoform β (PIPKIβ) in HL60 neutrophil migration.
In polarized neutrophils by treatment with chemoattractant, the PIPKIβ
concentrates on uropods. To produce this distribution, PIPKIβ requires its
C-terminal domain specific to the β isoform. Overexpression of a modified form
of PIPKIβ which was deleted domain alters the chemotactic capacity of
neutrophils. This anomaly is associated with a failure in the polarization of
RhoA, monomeric G protein that in the normal chemotactic response, focuses on
the uropods which controls the contraction of actomyosin. A similar phenotype is
obtained by reducing the role of PIPKIβ by RNAi. In conclusion, Manes and
colleagues propose to PIPKIβ as a new element in the complex signaling network
that the identity of uropod in leukocytes.
Nicotinic acetylcholine receptors are allotter membrane proteins that belong to a large family of ligand-gated ion channels that mediate transmission in the cholinergic system. Structurally, it consists of five subunits of one of the four types alpha, beta, gamma and delta, with the alpha subunit is composed of nine possible subtypes (alpha 1-9) and four beta (beta-1-4). The binding of agonists to these receptors generates a sequence of conformational changes resulting in channel opening. Previously, this team at the Institute of Neurosciences of a joint center of the Miguel Hernandez University and the CSIC had shown that a residue of the alpha-7 nicotinic receptor, the aspartame in position 266 of the connecting sequence between Tran membrane domains M2 and M3, intervenes in the relationship between agonist binding and channel opening.
The fate of a cell is determined by signals from their environment and their ability to respond to them. Some signaling pathways are involved in vital decisions during the development of organisms, this being an example of epithelial-mesenchymal transition (EMT), a transformation of epithelial cells into mesenchymal cells with migratory and invasive capacity in developing normal (in fact, crucial for the formation of different embryonic tissues, notably the mesoderm and neural crest), but also occurs in pathological conditions such as tumor progression in aggressive carcinomas. Read the rest of this entry »
Insulin is a paradigm of regulation and specific function. Essential anabolic hormone in the adult, seems to have a different role during embryonic development. A group from the Center for Biological Research, CSIC has been characterized step by step this new role. Insulin, as its precursor proinsulin and acting through insulin receptor hybrid with the growth factor insulin-like IGF-I, regulates the process of programmed cell death in the early stages of development of the nervous system stage where this process is, in turn, little studied. Read the rest of this entry »
Claudin-1 is an integral membrane protein of about 23 kDa, mapada on chromosome 3, whose function is cell adhesion. Is involved in cell growth and maintenance and is present in the tight junctions between cells, structures whose main functions emphasizes maintaining the differentiation state of epithelial cells. Recently it was reported that claudin may be, if not cause, itself a marker for the progression of certain carcinomas and metastases. Read the rest of this entry »
The cover image of the international edition of the February 13 issue of the journal Angewandte Chemie German represents a new form of DNA helices formed by three researchers who have described CSIC, Institute of Biomedical Research of the PCB and the University of Birmingham, in a collaboration that has found a surprising structure almost by chance, while trying to test the effectiveness of a drug as a therapeutic agent against DNA targets. Read the rest of this entry »