Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • images71After four years of postdoctoral fellowship at the Cold Spring Harbor Laboratory under the supervision of Carol Greider, the disciple of Margarita Salas, Maria Blasco returning to Spain in 1997 to join the National Center for Biotechnology, CSIC. There, Blasco intended to continue his research work that had enabled him to clone one of the telomerase genes and generated the first knockout mouse for this gene. Now in our country, it happened several works of broad impact on the development of animal models for studying the involvement of telomerase in cancer development and aging processes. Read the rest of this entry »

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    Chemotaxis is the movement which carried out certain cells in response to a
    chemical stimulus. In multicellular organisms, key cells are capable chemotactic
    leukocytes. This ability is essential for immune system function and homeostasis
    and its deregulation is associated with chronic inflammatory processes.

    The movement of leukocytes is the result of an asymmetric
    reorganization of their cytoskeleton: At the anterior end, the leading edge
    expands due to polymerization of actin-F, while in the uropod retracts back
    because of the contraction of actomyosin filaments. This reorganization is
    asymmetric, in turn, result in the polarization of different molecules and the
    complex signaling cascades that occur in response to chemical stimulation.
    Santos Manes’s team at the National Center of Biotechnology, CSIC has conducted
    various studies on the molecular mechanisms that cause this cell polarity. On
    this occasion, have investigated the role of the enzyme phosphatidylinositol
    4-phosphate 5-kinase type I isoform β (PIPKIβ) in HL60 neutrophil migration.

    In polarized neutrophils by treatment with chemoattractant, the PIPKIβ
    concentrates on uropods. To produce this distribution, PIPKIβ requires its
    C-terminal domain specific to the β isoform. Overexpression of a modified form
    of PIPKIβ which was deleted domain alters the chemotactic capacity of
    neutrophils. This anomaly is associated with a failure in the polarization of
    RhoA, monomeric G protein that in the normal chemotactic response, focuses on
    the uropods which controls the contraction of actomyosin. A similar phenotype is
    obtained by reducing the role of PIPKIβ by RNAi. In conclusion, Manes and
    colleagues propose to PIPKIβ as a new element in the complex signaling network
    that the identity of uropod in leukocytes.

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    Nicotinic acetylcholine receptors are allotter membrane proteins that belong to a large family of ligand-gated ion channels that mediate transmission in the cholinergic system. Structurally, it consists of five subunits of one of the four types alpha, beta, gamma and delta, with the alpha subunit is composed of nine possible subtypes (alpha 1-9) and four beta (beta-1-4). The binding of agonists to these receptors generates a sequence of conformational changes resulting in channel opening. Previously, this team at the Institute of Neurosciences of a joint center of the Miguel Hernandez University and the CSIC had shown that a residue of the alpha-7 nicotinic receptor, the aspartame in position 266 of the connecting sequence between Tran membrane domains M2 and M3, intervenes in the relationship between agonist binding and channel opening.

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  • snail-resisting-rather-than-dieThe fate of a cell is determined by signals from their environment and their ability to respond to them. Some signaling pathways are involved in vital decisions during the development of organisms, this being an example of epithelial-mesenchymal transition (EMT), a transformation of epithelial cells into mesenchymal cells with migratory and invasive capacity in developing normal (in fact, crucial for the formation of different embryonic tissues, notably the mesoderm and neural crest), but also occurs in pathological conditions such as tumor progression in aggressive carcinomas. Read the rest of this entry »

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  • new-regulation-for-a-particular-function-of-insulinInsulin is a paradigm of regulation and specific function. Essential anabolic hormone in the adult, seems to have a different role during embryonic development. A group from the Center for Biological Research, CSIC has been characterized step by step this new role. Insulin, as its precursor proinsulin and acting through insulin receptor hybrid with the growth factor insulin-like IGF-I, regulates the process of programmed cell death in the early stages of development of the nervous system stage where this process is, in turn, little studied. Read the rest of this entry »

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  • a2Claudin-1 is an integral membrane protein of about 23 kDa, mapada on chromosome 3, whose function is cell adhesion. Is involved in cell growth and maintenance and is present in the tight junctions between cells, structures whose main functions emphasizes maintaining the differentiation state of epithelial cells. Recently it was reported that claudin may be, if not cause, itself a marker for the progression of certain carcinomas and metastases. Read the rest of this entry »

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  • a1The cover image of the international edition of the February 13 issue of the journal Angewandte Chemie German represents a new form of DNA helices formed by three researchers who have described CSIC, Institute of Biomedical Research of the PCB and the University of Birmingham, in a collaboration that has found a surprising structure almost by chance, while trying to test the effectiveness of a drug as a therapeutic agent against DNA targets. Read the rest of this entry »

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