Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • RCAT is a highly sensitive and efficient amplification method that has been configured in a number of formats to address a broad range of applications.

    Linear RCAT (L-RCAT)
    One end of a DNA probe is joined to the desired target.
    The other end is hybridized to a generic DNA circle.

    An enzyme catalyst is added. Starting at the probe for the circle, the enzyme catalyst makes a copy of the DNA circle, returns to the starting point and continues to copy the circle.

    A single product is thus generated that is composed of copy after copy of the circle like a thread unraveling from a spool.
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  • Immunohistochemistry (IHC) and flow cytometry are highly respected technologies for the diagnosis of cancer and infectious disease. They are widely used by pathologists and increasingly used by large pharmaceutical and biotechnology companies to assist in target validation and the discovery of new drugs.
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  • Microspheres, also known as latex beads, polystyrene beads, and microbeads
    are a proven solid-phase format for development of assays used in both research
    and clinical diagnostics.

    Key advantages to bead format assays are:

    • Ease of manufacture
    • Faster assays; near–fluid-phase binding kinetics
    • Increased sensitivity; increased surface allows more antigen, antibody,
      or DNA to be included in the assay
    • Easily automated assay formats

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  • To diagnose disease, molecules characteristic of that disease must be studied, such as the DNA of a bacteria or virus, a protein produced by cancer cells, antibodies to an infection, or the sequence of a gene mutation. These may be present at very low, often undetectable levels.

    RCAT enables either the target or a signal attached to the target to be amplified in a way that allows detection of important biological markers that until now have been undetectable.
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  • Rolling Circle Amplification Technology (RCAT)
    is a proprietary amplification technology that offers multiple advantages
    to help meet the emerging needs of life sciences industries.

    RCAT: First-time performance advantages

    Expanded multiplexing
    for protein microarrays

    RCAT enables an unprecedented combination
    of sensitivity and multiplexing advantages for protein microarrays. This
    allows researchers for the first time to take advantage of the efficiency,
    throughput, and utility of microarrays for protein detection and measurement.

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  • RCAT is a highly sensitive and efficient amplification method that has been configured in a number of formats to address a broad range of applications.

    Linear RCAT (L-RCAT)

    One end of a DNA probe is joined to the desired target.
    The other end is hybridized to a generic DNA circle.

    An enzyme catalyst is added. Starting at the probe for the circle, the enzyme catalyst makes a copy of the DNA circle, returns to the starting point and continues to copy the circle.
    Read the rest of this entry »

    No Comments
  • The microarray is currently the format of choice for many approaches to
    genomics and pharmacogenomics and holds considerable promise as a platform
    for diagnostics and proteomics.

    Key advantages of microarrays are:

    • Massive multiplexing,
    • Multipurpose applicability,
    • Minute reagent consumption.

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