Molecular Staging

Most amplified strands are converted to double-stranded DNA at end of reaction.

Strand-displacement DNA synthesis proceeds.

With intervention of highly processive strand-displacing polymerase, hexamers anneal to genomic DNA target and prime DNA synthesis.

MDA is a molecular amplification method that enhances simple, rapid, isothermal whole-genome amplification.

By applying MDA to linear genomic DNA, it is possible to achieve a million-fold amplification with a very high degree of accuracy (bias 2-3 fold and greater than 99% coverage).

Hexamer primers are added to linear genomic DNA together with a highly specialized polymerase. The unique properties of the polymerase proceed to carry out strand displacement DNA synthesis on double- or single-stranded DNA templates. Once initial priming has occurred, an exponential cascade of branched amplification generates single-stranded templates for ongoing priming.
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Multiple Displacement Amplification (MDA) is a proprietary amplification technology that enables the first effective whole genome amplification method.

MDA enables for the first time:

The growing trend to study disease and drug response at the molecular level has focused attention on DNA as a precious resource. MDA technology enables the first effective, whole genome amplification method (W.G.A.) with the potential to transform the current practice of sample preparation and sample archiving for genetic testing.
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