Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • Initiation Apparatus Transcription Initiation Apparatus

    We discovered that the RNA polymerase II transcription initiation apparatus contains a multiprotein complex, called the Srb/mediator complex, which is involved in mediating the response to transcriptional activators and repressors. We have isolated genes encoding components of the yeast, murine and human Srb/mediator complexes and have used them to study the roles of specific Srb/mediator proteins in gene regulation . Read the rest of this entry »

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  • Subunit composition of Srb/Mediator-like complexes

    The subunits of yeast, human and mouse SRB/mediator-like complexes are compared. Identical or orthologous subunits are marked by tan boxes and connected by lines. Numbers indicate subunit size in kDa. Soh1 has not yet been confirmed as a component of the yeast Srb/Mediator. Read the rest of this entry »

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  • Several themes are featured. Transcriptional activators bind to specific DNA sequences, and the chromatin context of the DNA biding site can have a positive or negative influence on binding of the activator or the proteins it recruits to the promoter. Activators can bind and recruit chromatin remodeling and modifying complexes that influence local chromatin structure. Read the rest of this entry »

    Comments Off on A model for the role of activators in transcription initiation