Molecular Staging

Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.

  • images23Cancer, such as biological process that goes beyond the laws of development and cellular growth, requires relocation process and investigate molecular mechanisms that lead to uncontrolled cell division, genomic instability and tumor development. The identification of these new patterns of growth and development can be very important to understand and combat processes tumorogénesis.1 It appears from research such as referenced here, the Cdc6 and other cellular components are joined, forming complexes prerreplicativos before initiated DNA duplication. Read the rest of this entry »

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  • images83The known function until Protein securina is to prevent sister chromatids to separate prematurely before they are each anchored properly to spindle microtubules. This function is performed by the securina separate them interacting with the enzyme and inhibiting its proteolytic activity. Once the sister chromatids are properly arranged and anchored in the equatorial plane, the securina is ubiquitin and degraded, releasing to separate them, which then digests the cohesin Scc1. The Scc1 protein digestion results in the destabilization of the protein complex that holds the chromatids and allows their distribution to each of the cell poles. The premature separation of chromosomes can cause an abnormal distribution and occurrence of aneuploidy. Read the rest of this entry »

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  • images74La función conocida hasta el momento de la proteína securina es evitar que las cromátidas hermanas se separen prematuramente antes de estar cada una de ellas anclada correctamente a los microtúbulos del huso mitótico. Esta función la realiza la securina interaccionando con la enzima separasa e inhibiendo su actividad proteolítica. Una vez las cromátidas hermanas están dispuestas y ancladas correctamente en el plano ecuatorial, la securina se ubiquitiniza y se degrada, liberando a la separasa, que entonces digiere la cohesina Scc1. La digestión de la proteína Scc1 tiene como consecuencia la desestabilización del complejo proteico que mantiene unidas las cromátidas y permite la distribución de éstas a cada uno de los polos celulares. La separación prematura de las cromátidas puede originar una distribución anómala y la aparición de aneuploidías. Read the rest of this entry »

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  • images16A molecular biology laboratory come from clinical different types of samples, each with its own peculiarities to the analysis and obtain his DNA. “A priori” two different situations arise. If the aim is to know the physical location of the DNA / RNA of interest in a histological section shall be undertake studies in situ, whereas if it is not necessary to know that location is sufficient to obtain cellular DNA in solution. The DNA in solution is a mixture of mitochondrial DNA, eukaryotic nuclear and viral / bacterial if present in the sample virus / bacteria. Read the rest of this entry »

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  • The Chain Reaction (PCR) is a laboratory technique that permits copying in vitro of specific DNA sequences and has revolutionized the field of molecular biology. Conceptually, the PCR is a simple technique is to heat the separation of the two strands of DNA to be amplified is, and a copy simultaneously from a point determined by an artificial DNA fragment called primer, through the action of an enzyme called DNA polymerase. The result is a doubling of the number of molecules of a particular sequence of DNA. This process is repeated a number of times or cycles, such as 30, and achieved an increase or exponential amplification of the number of copies of template DNA fragment. Read the rest of this entry »

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  • images4The introduction of rapid methods of sequencing in the late ’70s represented a major shift in studies of two methods of DNA sequencing of nucleic acids. One of them (Maxam and Gilbert) chemical reagents used to cut DNA at specific base. The other is called enzymatic chain termination or dideoxy (Sanger, Nicklen and Coulson). The purpose of both is to get the whole sequence of each of the bases that form a nucleic acid fragment. Read the rest of this entry »

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  • images3Chemical molecules are attached to the probe and that will enable the detection of this after a process of hybridization. There are many types of reporter molecules: radioactive (32P, 35S), affinity (Biotin, Digoxigenin …), enzymatic (phosphatase, peroxidase …) and chemiluminescent (esters of acridine).

    What factors affect the sensitivity and specificity of the hybridization reaction? Read the rest of this entry »

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  • biomol21The introduction of rapid methods of sequencing in the late ’70s represented a major shift in studies of two methods of DNA sequencing of nucleic acids. One of them (Maxam and Gilbert) chemical reagents used to cut DNA at specific base. The other is called enzymatic chain termination or dideoxy (Sanger, Nicklen and Coulson). The purpose of both is to get the whole sequence of each of the bases that form a nucleic acid fragment. Read the rest of this entry »

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  • Hybridization solution: The probe and target nucleic acid are in a liquid. The hybridization conditions must be in order. The rate of formation of the duplex under these conditions is high. The problem with this type of hybridization is the elimination of unreacted probe, using among other methods nuclease S1-precipitation with trichloroacetic or hybridization protection assay. Read the rest of this entry »

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  • Few areas of molecular biology have not changed with the emergence of a number of techniques subsumed under the generic term for Genetic Engineering and interchangeably referred to as cloning, recombinant DNA or genetic manipulation. Before the development of Genetic Engineering was not possible to isolate a particular eukaryotic gene in sufficient quantities for study molecular or your product. Read the rest of this entry »

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