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  • When the elongation requires FACT

    resizeThe protein-coding genes in eukaryotes are transcribed by the activity of RNA polymerase II. This transcript has three distinct phases, the third of which is the elongation. In the process of elongation by RNA polymerase II is linked to mRNA processing and release of protein-mRNA complex into the cytoplasm. Many protein factors cooperate with RNA polymerase II in the assembly of all this machinery of transcription and elongation.

    Sebastian Chavez’s team at the University of Seville, has specialized in the development of tools for in vivo studies of this mechanism and the genetic analysis of these elements as a strategy to discern the functional relationships between them. Result of this work is the study discussed here, presented in Molecular and Cell Biology, and has helped to clarify some aspects of chromatin organization and the transcribed region-dependent role of these factors (the FACT complex).

    Apparently derived from recent results, the FACT complex stimulates elongation by interacting with nucleosomes and the polymerase. However, the requirement of FACT is not equally necessary for all genes. For example, inactivation of FACT subunits, Spt16 and affects the transcription of genes that have positioned nucleosomes in transcribed regions (GAL1, PHO5), whereas no influence so evident in genes with a random nucleosome structure. The findings of this study indicate that the requirement for FACT during transcription depends on the organization of chromatin in the 5 ‘end of the region being transcribed.

    Published on December 17, 2012 · Filed under: Bioscience; Tagged as: , , ,
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