Molecular Staging Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
  • Transcriptional profiles in yeast phosphatases

    images20The yeast Saccharomyces cerevisiae is probably the organism more closely associated with the progress, welfare and recent human evolution. The result of this importance in human activities, is one of the most studied organisms from their genomic sequence to the specific fermentative activity of the many existing varieties. These groups united by the study of this yeast is that of Joaquín Ariño and Antonio Casamayor and his team of molecular biology of yeast at the Autonomous University of Barcelona.

    The investigation of these specialists are reflected in a recent article in The Journal of Biological Chemistry on the transcriptional profiles of different phosphatases in Saccharomyces and functional role for them. Specifically, the study focuses on protein phosphatases 2C, a group of phosphatases encoded by several genes related. The experts analyzed transcriptional profiles in various mutants of these genes and identified two basic patterns, that of a mutation in the gene PTC1 and generated by deletion of genes PTC2-5.

    The mutation in PTC1 results in enhanced expression of genes that are also expressed when there is damage in the cell wall to maintain the integrity of it. There is also an increase in the activation of calcineurin and cell sensitivity to calcium, heavy metals and high pH, and a vacuolar function deficit. The authors speculate that the direct effect of loss of PTC1 is concrete action in a bad cell vacuolar activity and, therefore, the defects previously mentioned. This new perspective on the role of this gene could facilitate the search for specific protein targets PTC1 unidentified so far.

    Published on December 16, 2012 · Filed under: Bioscience; Tagged as: , ,
    Comments Off on Transcriptional profiles in yeast phosphatases