Molecular Staging Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
  • Cyclin regulatory mechanism: the key to the division 2

    images48The cells of any living being divided by two different mechanisms: through mitosis, which is the way in which cells are involved in normal cell growth in most living organisms, and through meiosis, the process by formed sex cells or germ.The meiotic cell cycle differs from the mitotic to have a phase called premeiotic DNA synthesis. This phase is characterized by high levels of recombination and two successive nuclear divisions. REM1 is a cyclin that is expressed only in meiosis in the fission yeast Schizosaccharomyces pombe.

    The gene encoding REM1 contains an intron with a stop codon in the reading frame, so to be processed (via splicing) to ensure it fully mature protein. REM1 messenger RNA is expressed throughout meiosis but its intron is processed only at the stage of meiosis I.
    The group at the Universitat Pompeu Fabra, led by Jose Ayte recently described in Nature, that the messenger RNA processing REM1 encodes a truncated protein of 17 kDa required for recombination levels are normal, whereas in meiosis I — intron-processed after the protein is expressed in mature, 56 kDa, with basic functions necessary for proper progression of meiosis.

    In this study we have determined, on a molecular scale, two transcription factors Forkhead family / Winged-helix regulate this process by binding both to the promoter of REM1. Fkh2, prevents the processing of messenger RNA REM1 during the premeiotic DNA synthesis, while promoting Mei4 intron processing in meiosis I. The process is carried out by a physical interaction with Mei4 running the splicing machinery (spliceosomes).

    Published on November 15, 2012 · Filed under: Bioscience; Tagged as: , , ,
    Comments Off on Cyclin regulatory mechanism: the key to the division 2