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  • Bioactive endogenous jasmonate

    melissa-pvc-sandalia4The group of Roberto Solano, Department of Plant Molecular Genetics at the National Center for Biotechnology, made the study of signaling pathway of the phytohormone jasmonate (JA) in Arabidopsis thaliana and the molecular mechanisms underlying the activation, plant defenses against pathogens.

    It is known that in the perception of JA requires two proteins, both protein coronatina insensitive 1 (COI1) and the JAZ repressor proteins (jasmonate ZIM domain). In a first step regulator, the precursor of JA enzyme binds to molecules such as L-Ile jasmonoil producing the active ligand-isoleucine(JA-Ile). The formation of JA-Ile complex with these proteins leads to ubiquitination and degradation of JAZ proteins allowing, then, the transcription of genes involved in plant development and defense against pathogens.

    It remained to decide what form of JA was the real bioactive hormone in JA signaling. After testing in vitro and in vivo hormonal activity of a series of natural and synthetic analogs of JA, show that, contrary to what was coming to believe so far, the bioactive hormone is the(+)-7-iso-jasmonoil-L-isoleucine, structurally similar to the phytotoxin coronatina. Furthermore, in A. thaliana, the COI1 is the recipient of both coronatina as JA-L-Ile. Moreover, the form(-)-7-JA-L-Ile is inactive. It is suggested that the epimerization or interconversion of the molecule at(-)-JA-L-Ile is a possible mechanism for regulation of hormonal response.

    Published on November 9, 2012 · Filed under: Bioscience; Tagged as: , ,
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