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  • THEORETICAL BASES OF THE ART LCR

    images20The basic principle of any molecular diagnostic technology is the detection of a specific sequence of nucleic acids by hybridization with a complementary sequence, the probe, followed by the detection of the hybrid. However, the sensitivity of tests using nucleic acid probes without resorting to amplification is lower than the classical tests. Its primary application is the identification of microorganisms rather than detection.

    DNA replication was possible in 1958 when Kornberg discovered DNA polymerase, for many years was the primary application of DNA sequencing (Sanger et al.). In 1986, Mullis et al. described an iterative process that allowed exponentially increase the number of nucleic acids (amplification). amplification techniques can be classified in different ways: conceptually, those in which the target is amplified (polymerase chain reaction-PCR-, strand-displacement amplification – SDA, “nucleic acid sequence-based amplification, NASBA-, transcription-mediated amplification-TMA-), the probe is amplified (amplification-QßRA QSS-replicate) and mixed (gapped-LCR).

    Published on October 29, 2012 · Filed under: Bioscience; Tagged as: , , , ,
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