Molecular Staging Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
  • Circle AmplificationTM in Detecting Allergen-specific IgE on Microarrays Published

    NEW HAVEN, CT., October 30, 2001/– Molecular Staging Inc. (MSI) today announced a published abstract in the October 2001 issue of Clinical Chemistry. The abstract described the application its of MSI’s Rolling Circle Amplification TechnologyTM (RCATTM) for the detection of allergen-specific IgE, the antibody in patient serum used to predict an allergic response in individuals with concordant clinical history.

    Through pPreviously published studies , it is understoodhave demonstrated that MSI’s RCAT has an ultra-sensitive ability for detecting and measuring proteins. RCAT is an amplification technology that uniquely allows amplification and detection of proteins directly on a microarray. This microarray technology offers a number of advantages over existing allergy testing methods such as skin prick testing and laboratory based blood testing. The procedure requires less costs and time than the standard, and is better tolerated by patients with the use of the RCAT technology. Microarray technology can screen hundreds of allergens simultaneously, use minute amount of expensive allergens, and use non-invasive procedures to minimize patient discomfort. Microarray technology also has a quantitative ability to test, measure, and monitor the allergic response results to immunotherapy techniques over time.

    This microarray based method offers a number of advantages over existing allergy testing methods such as skin prick testing and laboratory based blood testing.

    In particular, this innovative approach:

    · Enables screening of hundreds of allergens simultaneously

    · Uses minute amounts of expensive allergens

    · Is non-invasive, i.e. minimizes patient discomfort

    · Is quantitative for enabling testing of allergic response over time

    The abstract in Clinical Chemistry is entitled “Allergen-specific IgE Detection on Microarrays Using Rolling Circle Amplification: Correlation with in In Vitro Assays for Serum IgE.” In this study, researchers compared results obtained by RCAT, termed “immunoRCA”, on microarrays with those obtained from two commercially available antigen-specific IgE assays and one in-house reference laboratory test. Forty-four serum samples were selected, providing six positive and five negative serum samples for cat dander, dust mites, short ragweed, and peanuts. Results indicate that the immunoRCA RCAT microarray assay correlates with alternative scoring method assigned using the Pharmacia CAP assay and other commercial assays. The RCAT system was beneficial because of its multiplexing ability, which allows scientists to look at hundreds of allergens simultaneously.

    “We anticipate that multiplexed allergen detection on microarrays using RCAT will provide physicians with quantified results and more information in a rapid time frame whilst improving the quality of patient care,” said MSI’s Chief Operating Officer Stephen Kingsmore, M.D.

    MSI is a life science company developing a portfolio of technologies for detection and measurement of proteins and nucleic acids across a broad range of applications, including proteomics, genomics, pharmacogenomics and diagnostics. One of MSI’s core technologies is Rolling Circle Amplification Technology (RCATTM), an amplification process that has significant advantages in terms of sensitivity, multiplexing, dynamic range and scalability. MSI has exclusively licensed RCATTM from Yale University. Additional information about MSI can be found at www.molecularstaging.com.

    Published on October 7, 2012 · Filed under: Press Release;
    Comments Off on Circle AmplificationTM in Detecting Allergen-specific IgE on Microarrays Published