Molecular Staging Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
  • RCATTM Technology Overview

    To diagnose disease, molecules characteristic of that disease must be studied, such as the DNA of a bacteria or virus, a protein produced by cancer cells, antibodies to an infection, or the sequence of a gene mutation. These may be present at very low, often undetectable levels.

    RCAT enables either the target or a signal attached to the target to be amplified in a way that allows detection of important biological markers that until now have been undetectable.

    RCAT uses a circular DNA template and a highly specialized polymerase to generate a long, single-strand DNA product comprising thousands of copies of the circle. Along this single strand of DNA are binding sites for thousands of fluorescent dyes. The brightly labeled strand remains attached or “tethered” to the target molecule or analyte, facilitating detection. This increases precision, allows multiple experiments to be conducted simultaneously (multiplexing), and encourages economy of scale and the minimal use of expensive reagents, such as monoclonal antibodies or enzymes.

    It is the only practical amplification method that allows recognition, amplification, and detection of targets directly on a solid surface, such as within a cell, or on a microarray or biochip, and in an isothermal environment.

    Published on September 29, 2012 · Filed under: RCAT;
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