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  • MDA Advantages

    Multiple Displacement Amplification (MDA) is a proprietary amplification technology that enables the first effective whole genome amplification method.

    MDA enables for the first time:

    Whole Genome Amplification with enhanced coverage and minimal bias.

    The growing trend to study disease and drug response at the molecular level has focused attention on DNA as a precious resource. MDA technology enables the first effective, whole genome amplification method (W.G.A.) with the potential to transform the current practice of sample preparation and sample archiving for genetic testing.


    The MSI MDA technology platform enables:

    • A virtually unlimited number of tests per sample
    • Use of smaller samples, such as buccal swabs, mouthwash, and finger prick
    • Use of DNA from alternative sources, such as biopsies and needle aspirates
    • An increase in the practice of archiving DNA for additional testing or re-testing
    • Amplification directly from whole blood and cells

    MDA is a substantial advancement over currently available W.G.A. methods, which are based on PCR. The MDA method rapidly amplifies the genome with comprehensive loci coverage and minimal bias between any loci, yielding 10+kb fragments in a simple, scalable reaction.

    Optimal coverage and minimal bias

    Comprehensive coverage of all single-copy gene sequences with unprecedented low bias offers researchers the confidence needed for an expanded range of downstream applications.

    Accuracy
    Use of enzyme with proofreading capability yields amplified material true to the original material.

    This enables researchers to perform assays, such as SNP assays, where accuracy is paramount.

    Scalable

    Larger quantities of DNA can be generated simply by increasing the reaction volume. A 1ml reaction gives the same DNA yield per µl as a standard 100µl reaction. This ability to carry out large volume reactions enables large-scale studies such as high-density genotyping.


    Sample prep step eliminated

    The MDA whole genome amplification method can be carried out directly on crude lysed cells, whole blood, buccal swabs, and other biological samples, eliminating the costly and time-consuming DNA purification step.

    Assay-ready

    Contaminants are effectively diluted out during the MDA process, replacing the need for DNA
    purification steps and allowing downstream applications, such as TaqMan®,
    to be carried out with no inhibitory effects.

    High throughput
    Ideally suited for 96-well-plate automated systems, with no centrifugation and/or filtrations needed.

    Long Fragments
    The MDA process generates DNA in excess of
    10kb in length, which enables traditional molecular biology methods such
    as RFLP.

    TaqMan is a registered trademark of Applied Biosystems.

    VITAL CHARACTERISTICS:
    Sample Targets
    • Genomic DNA, whole blood, buffy coats,
      buccal swabs, mouthwash, and cultured cells
    Amount
    • 1µl blood, single cheek swab, sub-ng gDNA
    Lysis Procedures
    • 10-minute one-step lysis
    Yield
    • 50µg DNA from a few genomic copies
      in a 100µl reaction

    • Scalable to 10mg DNA yield from
      large-scale reaction
    • Consistent yield independent of amount
      of starting sample

    Reaction Time
    • 6 hours or overnight
    Coverage
    • All loci represented at approximately

      same level as in starting genomic DNA target,
      no more than 6-fold difference

    • Only highly repetitive sequences lost, such as
      centromere and telomere repeats
    Assay-ready DNA
    • PCR-based assays, such as SBE, TaqMan,
      restriction enzyme digestion, and STRs

    • Southern analysis, including RFLP
    • DNA sequencing
    Published on September 23, 2012 · Filed under: MDA Technology; Tagged as:
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