Molecular Staging Molecular Staging is addressing this demand with a portfolio of products and services based on technologies that are transforming the detection and measurement of both proteins and nucleic acids.
  • Protein Separation

    Protein analysis uses a diseased or treated sample and a control sample. 2D gel electrophoresis is performed for each sample to separate proteins based on their molecular weight and charge.

    Reproducibility

    One of the key developments in proteomics was the development of gels which deliver reproducible results. In a reproducibility experiment, three cultures of the same Staphylococcus aureus strain were grown to stationary phase. Protein was extracted from each and separated by 2D gel electrophoresis at Oxford GlycoSciences (OGS).

    Black marks on the gel images indicate a protein or cluster of proteins and are referred to as “features.” The x-axis is the Isoelectric point (pI) which is analagous to pH, while the y-axis is molecular weight (Mw) or size. The average feature variance taking in gel-to-gel and biological triplicate was an impressive 23% indicating strong reproducibility, even across different cultures of the same strain. As you can see, the gel images are very similar.

    The breakthroughs made by OGS in creating an accurate and reliable electophoresis system now enable tissue-specific gel signatures to be generated. These gel signatures display a range of proteins expressed for any cell type. Incyte and OGS are using this technology platform to determine the protein profiles of different tissues in both healthy and disease states, working toward the creation of the LifeProtâ„¢ proteome library.

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    Published on September 18, 2012 · Filed under: Genetics; Tagged as: , ,
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